Establishment and Characterization of Feline Mammary Tumor Patient-Derived Xenograft Model

Feline mammary tumor is a relatively commonly diagnosed neoplasm in the cat. Development of new veterinary cancer therapies limited by shortage vivo models that reproduce microenvironment and metastatic progression. Four feline orthotopic patient-derived xenograft model (PDX) successfully established NOD-SCID gamma (NSG) mice. The overall success rate PDX establishment was 36% (4/11). Histological, immunohistochemical, short tandem repeat analysis showed remarkable similarity between patient’s xenograft. grafts conserve original essential features, including distant metastasis. Primary FMT-1807 cell line isolated from FMT-1807PDX tissue. Tumorigenicity cells expanded assessed injection into NSG Mice yielded tumors which preserve lung liver metastasis ability. This work provides platform for FMT translational investigation.